Hidden in pathology archives and biospecimen repositories around the world is a treasure trove of information locked within formalin-fixed parafin-embedded (FFPE) tissue samples.

FFPE archives constitute a huge library of clinically appraised specimens, whose analysis on the molecular level can be directly related to progression of a disease or therapy.

This user manual provides guidelines and protocols for the proper preparation and pretreatment of formalin-fixed, paraffin-embedded (FFPE) tissues mounted on slides.

FFPE tissue blocks and slides submitted for translational medicine or biobanking will not be returned. If the tissue is needed for participant care, approval for release of the FFPE tissue block or slides must …

Biospecimens processed under these procedural guidelines are suitable for analysis by polymerase chain reaction (PCR), methylation analysis, Sanger sequencing and next-generation sequencing, …

Frozen tissues that are correctly frozen (with OCT compound in a dry ice/isopentane slurry) are cryosectioned in a cryostat similar to the one shown in the photograph below. Frozen sections are …

Formalin-fixed paraffin-embedded (FFPE) tissue is commonly used in clinical oncology research. Before nucleic acid can be extracted from FFPE samples, they must be deparaffinized.