Gene expression experiments measure changes in RNA transcript levels to compare how genes are up- or down-regulated across ...

All real-time PCR systems rely upon the detection and quantitation of a fluorescent reporter, the signal of which increases in direct proportion to the amount of PCR product in a reaction. The ...

Polymerase Chain Reaction (PCR) is a method which utilizes the mechanism of DNA replication to amplify a specific part of DNA. This involves the use of DNA polymerase, which makes new strands of DNA ...

Although 5'-3' nuclease oligoprobe (TaqMan assay) is the most widely used format owing to its commercial utility and higher specificity as a result of sequence-specific hybridization of the probe, the ...